Free access
Issue
Apidologie
Volume 22, Number 6, 1991
Evolution and genetics
Page(s) 611 - 619
DOI http://dx.doi.org/10.1051/apido:19910604
Apidologie 22 (1991) 611-619
DOI: 10.1051/apido:19910604

Location of genes in Apis mellifera scutellata-derived mitochondrial DNA of Africanized honey bees

M.C. Ariasa and F.G. Nobregab

a  Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Genética, 14049 Ribeirão Preto, São Paulo, SP, Brazil
b  Universidade de Sâo Paulo, Instituto de Biociências, Departamento de Biologia, Caixa Postal 11461, 05499 São Paulo, SP, Brazil

Abstract - The mitochondrial DNA of Africanized honey bees having a mtDNA haplotype known from the African subspecies Apis mellifera scutellata, was analysed for the location of 6 mitochondrial genes by hybridization to mitochondrial gene probes from Saccharomyces cerevisiae. Genes for the small and large ribosomal RNAs, cytochrome oxidase subunits I, II and III and apocytochrome b were positioned relative to the mitochondrial DNA fragments generated with 6 different restriction endonucleases utilized singly or in combination. Results show that these genes are in the same relative positions as Drosophila genes.


Key words: Africanized honey bee / mitochondrial gene / gene mapping / restriction endonuclease / molecular hybridization