Location of genes in Apis mellifera scutellata-derived mitochondrial DNA of Africanized honey beesM.C. Ariasa and F.G. Nobregab
a Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Genética, 14049 Ribeirão Preto, São Paulo, SP, Brazil
b Universidade de Sâo Paulo, Instituto de Biociências, Departamento de Biologia, Caixa Postal 11461, 05499 São Paulo, SP, Brazil
Abstract - The mitochondrial DNA of Africanized honey bees having a mtDNA haplotype known from the African subspecies Apis mellifera scutellata, was analysed for the location of 6 mitochondrial genes by hybridization to mitochondrial gene probes from Saccharomyces cerevisiae. Genes for the small and large ribosomal RNAs, cytochrome oxidase subunits I, II and III and apocytochrome b were positioned relative to the mitochondrial DNA fragments generated with 6 different restriction endonucleases utilized singly or in combination. Results show that these genes are in the same relative positions as Drosophila genes.
Key words: Africanized honey bee / mitochondrial gene / gene mapping / restriction endonuclease / molecular hybridization