Free Access
Issue
Apidologie
Volume 32, Number 4, July-August 2001
Page(s) 323 - 332
DOI https://doi.org/10.1051/apido:2001132
DOI: 10.1051/apido:2001132

Apidologie 32 (2001) 323-332

Mitochondrial DNA characterization of five species of Plebeia (Apidae: Meliponini): RFLP and restriction maps

Flávio de Oliveira Francisco, Daniela Silvestre and Maria Cristina Arias

Departamento de Biologia, Instituto de Biociências, USP, São Paulo, SP, 05508-900, Brazil

(Received 15 September 2000; revised 12 March 2001; accepted 30 March 2001)

Abstract
The present work characterized the mitochondrial DNA (mtDNA) of five species of Plebeia ( Plebeia droryana , P. emerina, P. remota, P. saiqui and P. sp.) and generate a data set to be used in further populational, phylogenetic, and biogeographic studies. The mtDNA of each species was analyzed using 17 restriction enzymes and restriction maps were built. A high level of interspecific variability was found. The total size of the mtDNA was estimated to be 18500 bp. Through a combination of PCR and examination of restriction fragment length polymorphism, the locations of 14 of the main mitochondrial genes were located on restriction maps. We verified a gene order identical to Apis mellifera .


Key words: Plebeia / stingless bee / mtDNA / rflp / restriction map

Correspondence and reprints: Maria Cristina Arias
    e-mail: mcarias@ib.usp.br

© INRA, EDP Sciences, DIB, AGIB 2001

Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.

Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.

Initial download of the metrics may take a while.