Non-radioactive in-situ hybridisation to honeybee embryos and ovariesPeter Osborne and Peter K. Dearden
Laboratory for Development and Evolution, Biochemistry Department, University of Otago, Dunedin, New Zealand
(Received 5 July 2004 - Revised 23 August 2004 - Accepted 26 August 2004; Published online: 16 March 2005)
Abstract - To study the function and expression of genes identified in the honeybee genome sequence, techniques for assaying gene expression need to be developed. We present a robust method for preparing honeybee embryos and ovaries for in-situ hybridisation. This protocol should prove useful for researchers wishing to use the genome sequence to study honeybee biology.
Key words: Apis mellifera / embryo / ovary / gene expression / in-situ hybridisation
Corresponding author: Peter K. Dearden email@example.com
© INRA, DIB-AGIB, EDP Sciences 2005