Microsatellites for the inference of population structures in the Red Mason bee Osmia rufa (Hymenoptera, Megachilidae)Karsten Neumanna and Karsten Seidelmannb
a Martin-Luther-Universität Halle-Wittenberg, Institut für Zoologie, Abteilung Allgemeine Zoologie, Domplatz 4, 06099 Halle (Saale), Germany
b Martin-Luther-Universität Halle-Wittenberg, Institut für Zoologie, Abteilung Tierphysiologie, Domplatz 4, 06099 Halle (Saale), Germany
(Received 3 March 2005 - accepted 30 June 2005 - published online 10 January 2006)
Abstract - Microsatellite loci were isolated from the solitary Red Mason bee (Osmia rufa) by an enrichment protocol for partial genomic libraries. Six polymorphic microsatellite loci were used for a first population structure survey including 9 continental European and one island population. Observed levels of genetic variability and heterozygosity proved to be moderate. There was no significant differentiation among continental O. rufa populations. Only the island population from Cyprus was clearly separated. A correlation between geographical and genetic distance indicates gene flow among continental bees. The relatively high homogeneity in O. rufa is probably caused by low effective population size rather than an imprint of past population events. All microsatellites amplified in three further Osmia species.
Key words: microsatellites / genetic diversity / population structure / Osmia rufa
Corresponding author: Karsten Neumann firstname.lastname@example.org
© INRA, DIB-AGIB, EDP Sciences 2006