Issue Apidologie Volume 35, Number 4, July-August 2004 Page(s) 365 - 370 DOI 10.1051/apido:2004027

Apidologie 35 (2004) 365-370
DOI: 10.1051/apido:2004027

Mitochondrial DNA restriction and genomic maps of seven species of Melipona (Apidae: Meliponini)

Ricardo Weinlich, Flávio de Oliveira Francisco and Maria Cristina Arias

Departamento de Biologia, Instituto de Biociências, USP, São Paulo, SP, 05508-900, Brazil

(Received 27 December 2002; revised 3 September 2003; accepted 19 September 2003)

Abstract - This paper reports efforts to characterize the mitochondrial genome of Meliponini. Here we describe the restriction and partial genomic map of seven Melipona species (M. bicolor, M. compressipes, M. marginata, M. melanoventer, M. quadrifasciata, M. rufiventris and M. subnitida). The maps were obtained through RFLP and PCR-RFLP using 15 restriction enzymes. The total number of sites mapped ranged from 12 to 19, indicating a high level of genetic diversity among those species. MtDNA total size was estimated to be 18 500 bp.

Key words: Melipona / stingless bee / mtDNA / rflp / restriction map

Corresponding author: Maria Cristina Arias mcarias@ib.usp.br

© INRA, EDP Sciences, DIB, AGIB 2004

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