Study of the depletion of tylosin residues in honey extracted from treated honeybee (Apis mellifera) colonies and the effect of the shook swarm procedure

Free access article

Issue		Apidologie Volume 38, Number 4, July-August 2007


Page(s)		315 - 322
DOI		http://dx.doi.org/10.1051/apido:2007017
Published online		10 October 2007

Apidologie 38 (2007) 315-322
DOI: 10.1051/apido:2007017

Study of the depletion of tylosin residues in honey extracted from treated honeybee (Apis mellifera) colonies and the effect of the shook swarm procedure

Stuart J. Adams^a, Katharina Heinrich^a, Mike Hetmanski^a, Richard J. Fussell^a, Selwyn Wilkins^b, Helen M. Thompson^b and Matthew Sharman^a

^a Food Safety Group, Central Science Laboratory, Sand Hutton, York, YO41 1LZ, UK
^b National Bee Unit, Central Science Laboratory, Sand Hutton, York, YO41 1LZ, UK

(Received 5 July 2006 - Revised 7 December 2006 - Accepted 22 December 2006 - Published online 10 October 2007)

Abstract - Bee colonies were dosed with tylosin tartrate 1.1 g per hive (single dose in sucrose solution) and samples of honey were then collected at intervals over a 20-week period. The samples were analysed for tylosin A and desmycosin (tylosin B) using LC-MS/MS. The mean concentration of tylosin A in the honey (pooled results) 3 days after dosing was 17 $\mu$ g/g, declining to 0.9 $\mu$ g/g after 140 days. The mean concentration of desmycosin was 2.3 $\mu$ g/g, 3 days after dosing declining to 1.1 $\mu$ g/g after 140 days. The shook swarm procedure was investigated and resulted in a tylosin A concentration in brood honey of 10 $\mu$ g/g, 3 days after dosing declining to 0.02 $\mu$ g/g, 140 days after dosing. A corresponding decrease in the mean concentrations of desmycosin in brood honey, 1.1 $\mu$ g/g, 3 days after dosing to 0.03 $\mu$ g/g, 140 days after dosing also was observed. Tylosin A depletes to desmycosin in honey and can still be detected 238 days after dosing. Thus a more accurate residue definition is the sum of tylosin A and desmycosin.

Key words: Tylosin / desmycosin / honey / veterinary drug / residues / apiculture

© INRA, DIB-AGIB, EDP Sciences 2007

What is OpenURL?

The OpenURL standard is a protocol for transmission of metadata describing the resource that you wish to access. An OpenURL link contains article metadata and directs it to the OpenURL server of your choice. The OpenURL server can provide access to the resource and also offer complementary services (specific search engine, export of references...). The OpenURL link can be generated by different means.

If your librarian has set up your subscription with an OpenURL resolver, OpenURL links appear automatically on the abstract pages.
You can define your own OpenURL resolver with your EDPS Account. In this case your choice will be given priority over that of your library.
You can use an add-on for your browser (Firefox or I.E.) to display OpenURL links on a page (see http://www.openly.com/openurlref/). You should disable this module if you wish to use the OpenURL server that you or your library have defined.