Nucleotide sequence variations in Kashmir bee virus isolated from Apis mellifera L. and Varroa jacobsoni Oud.

Free access article

Issue		Apidologie Volume 31, Number 1, January-Febuary 2000


Page(s)		17 - 23
DOI		10.1051/apido:2000101

DOI: 10.1051/apido:2000101

Apidologie 31 (2000) 17-23

Nucleotide sequence variations in Kashmir bee virus isolated from Apis mellifera L. and Varroa jacobsoni Oud.

Akey C.F. Hung ${^{\rm a}}$ , Christine Y.S. Peng ${^{\rm b}}$ , H. Shimanuki ${^{\rm a}}$

${^{\rm a}}$ Bee Research Laboratory, USDA-ARS, Beltsville, MD 20705, USA
${^{\rm b}}$ Department of Entomology, University of California, Davis, CA 95616, USA

(Received 14 June 1999; accepted 19 August 1999)

Abstract:

The 374 bp amplicon of 20 Kashmir bee virus (KBV) isolates from 14 bee and 6 mite samples from California was sequenced. The sequence data and those of homologous KBV amplicons of one bee each from Australia, Canada, Maine, and Maryland were analyzed. Pairwise comparisons of nucleotide sequences show the mean distance between Californian isolates varying from 0% to 3.2%. The mean distance between Californian isolates and the two U.S. or the Canadian isolates are equal or less than 3.2%. The Australian isolate is different from all North American isolates with mean distances of 11.5 to 12.3%. This 374 bp sequence contains a single open reading frame, the 124 amino acid sequence matched closely with the sequences of many virus polyproteins.

Kasmir bee virus / direct RT-PCR / pairwise comparison / phylogenetic tree

Correspondence and reprints: Akey C.F. Hung
ahung@asrr.arsusda.gov

What is OpenURL?

The OpenURL standard is a protocol for transmission of metadata describing the resource that you wish to access. An OpenURL link contains article metadata and directs it to the OpenURL server of your choice. The OpenURL server can provide access to the resource and also offer complementary services (specific search engine, export of references...). The OpenURL link can be generated by different means.

If your librarian has set up your subscription with an OpenURL resolver, OpenURL links appear automatically on the abstract pages.
You can define your own OpenURL resolver with your EDPS Account. In this case your choice will be given priority over that of your library.
You can use an add-on for your browser (Firefox or I.E.) to display OpenURL links on a page (see http://www.openly.com/openurlref/). You should disable this module if you wish to use the OpenURL server that you or your library have defined.