A Nosema ceranae isolate from the honeybee Apis mellifera

Free access article

Issue		Apidologie Volume 38, Number 1, January-February 2007


Page(s)		30 - 37
DOI		10.1051/apido:2006054
Published online		16 January 2007

Apidologie 38 (2007) 30-37
DOI: 10.1051/apido:2006054

A Nosema ceranae isolate from the honeybee Apis mellifera

Wei-Fone Huang^a, Jing-Hao Jiang^a, Yue-Wen Chen^b and Chung-Hsiung Wang^a

^a Department of Entomology, National Taiwan University, Taipei 106, Taiwan
^b Department of Animal Science, National I-Lan University, I-Lan 260, Taiwan

(Received 19 December 2005 - Revised 18 April 2006 - Accepted 4 May 2006 - Published online 16 January 2007)

Abstract - Microsporidiosis (nosema disease) of the honeybee, Apis mellifera, has spread worldwide and caused heavy economic losses in apiculture. We obtained a spore isolate from worker ventriculi of A. mellifera colonies kept on the campus of National Taiwan University and sequenced the ribosomal genes. The entire length of the ribosomal DNA is about 3828 bp and the organization is similar to that of Nosema apis. However, the SSUrRNA, ITS, and LSUrRNA sequences have comparatively low identities with those of N. apis (92, 52, and 89%, respectively) and the SSUrRNA has a 99% identity with Nosema ceranae. These results indicate that this isolate is not N. apis, but N. ceranae. Moreover, the morphological characteristics are identical to those of N. ceranae. These results show that nosema disease of the honeybee, A. mellifera, may not be caused solely by the infection of N. apis.

Key words: Nosema ceranae / rRNA / nosema disease / Microsporidia

Corresponding author: wangch@ntu.edu.tw

© INRA, DIB-AGIB, EDP Sciences 2007

What is OpenURL?

The OpenURL standard is a protocol for transmission of metadata describing the resource that you wish to access. An OpenURL link contains article metadata and directs it to the OpenURL server of your choice. The OpenURL server can provide access to the resource and also offer complementary services (specific search engine, export of references...). The OpenURL link can be generated by different means.

If your librarian has set up your subscription with an OpenURL resolver, OpenURL links appear automatically on the abstract pages.
You can define your own OpenURL resolver with your EDPS Account. In this case your choice will be given priority over that of your library.
You can use an add-on for your browser (Firefox or I.E.) to display OpenURL links on a page (see http://www.openly.com/openurlref/). You should disable this module if you wish to use the OpenURL server that you or your library have defined.