Isolation and characterization of Bacillus coagulans associated with half-moon disorder of honey beesJ.D. Vandenberg and H. Shimanuki
USDA-ARS Beneficial Insects Laboratory, Beltsville, MD, 20705, USA
Abstract - Nine different isolates of the bacterium Bacillus coagulans were isolated from honey bee larval cadavers collected from colonies afflicted with half-moon disorder (HMD) in New Zealand. One isolate was selected for detailed characterization and pathogenicity testing. Laboratory-reared larvae became infected when inoculated with the test isolate at 1 or 2 d of age. No infection was observed among larvae inoculated in combs placed in small colonies in outdoor cages. Although able to infect larvae under certain conditions, B coagulans is probably not the cause of HMD.
Key words: Bacillus coagulans / Apis mellifera / honey bee larvae / half-moon disorder