Resistance of Bacillus larvae in beeswaxM. Máchová
Bee Research Institute, Dol 252 66 Libice nad Vltavou, Czech Republic
Abstract - A simple routine microbiological method was used to determine the titre of viable Bacillus larvae spores in beeswax. Viable bacillus larvae spores in beeswax were tested for heat resistance at the constant temperature of 150 °C and for acid-resistance to 0.5% H2SO4. Heat resistance of Bacillus larvae spores in beeswax medium was expressed by the index D150 °C, ranging from 1.5 - 2.5 min depending on the initial concentration of spores. 0.5% H2SO4 did not result in the reduction of the number of viable Bacillus larvae spores for the 37.5 h required for the isolation of spores from beeswax.
Key words: Bacillus larvae spore / beeswax / heat resistance / acid-resistance / disinfection