Free access
Volume 24, Number 2, 1993
Page(s) 89 - 94
Apidologie 24 (1993) 89-94
DOI: 10.1051/apido:19930201

Isolation and identification of linoleic acid as an antimicrobial agent from the chalkbrood fungus, Ascosphaera apis

M.F. Feldlaufera, W.R. Lusbya, D.A. Knoxb and H. Shimanukib

a  Insect Neurobiology and Hormone Laboratory, Bldg, 467 BARC-East, Beltsville, MD, USA
b  Bee Research Laboratory, Bldg 476 BARC-East, Beltsville MD 20705, USA

Abstract - Ethanol extracts of mixtures of mycelia and spores of Ascosphaera apis, the causative agent of chalkbrood disease in honey bee, was shown to contain an antimicrobial compound active against Bacillus larvae, the causative agent of American foulbrood disease. Purification by high performance liquid chromatography and analysis by mass spectrometry identified the active compound as 9,12-octadecadienoic acid (linoleic acid).

Key words: chalkbrood / American foulbrood / antimicrobial / linoleic acid / Bacillus larvae