Asymmetrical distribution of a mitochondrial DNA polymorphism between 2 introgressing honey bee subspeciesM.D. Meixnera, W.S. Sheppardb and J. Poklukarc
a Institut für Bienenkunde (Polytechnische Gesellschaft), JW Goethe Universität, Frankfurt am Main, Karl-von-Frisch-Weg 2, 6370 Oberursel, Germany
b USDA-ARS, Bee Research Laboratory, Bldg 476, BARC-E, Beltsville, MD 20705, USA
c Agricultural Institute of Slovenia, Hacquetova 2, 61000 Ljubljana, Slovenia
Abstract - Mitochondrial DNA from 62 samples of Apis mellifera carnica and A m ligustica collected from within areas of endemism and an area of known introgression was extracted and digested with the restriction enzymes Accl, Bc/l, Bg/II, EcoRI and Xbal. Digestion with Xbal revealed a polymorphism with an asymmetrical distribution between the subspecies. This polymorphism occurs primarily in Austrian and Slovenian A m carnica and may be useful for studying introgression between this race and A m ligustica.
Key words: Apis mellifera / mitochondrial DNA / subspecies / population genetics