Apidologie 31 (2000) 167-180
Molecular confirmation of a fourth lineage in honeybees from the Near East
aLaboratoire de Modélisation et Biologie Evolutive, URLB-INRA,
488 rue Croix de Lavit, 34090 Montpellier, France
bLaboratoire Population, Génétique et Évolution, CNRS, Gif-sur-Yvette, France
The mitochondrial DNA (mtDNA) from 75 honeybee colonies from the Lebanon was characterized by DraI
restriction fragment length polymorphism (RFLP) of the COI-COII intergenic region. The seven observed
haplotypes were different enough from all haplotypes already known in Apis mellifera to justify
their assignment to a fourth mtDNA lineage. The nucleotide sequence of a 380 base pair (bp) fragment of
the NADH2 gene was determined for two haplotypes, which showed a high similarity with two published
sequences from A. m. lamarkii and A. m. meda. A microsatellite analysis of a large Lebanese
population sample (50 colonies, 8 loci) suggests that Near East populations are also differentiated at the
nuclear level from the three previously characterized evolutionary branches of the species
Apis mellifera / mtDNA / microsatellite / evolutionary history / Near East
Correspondence and reprints: Jean-Marie Cornuet
Copyright INRA/DIB/AGIB/EDP Sciences
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