Free access
Volume 31, Number 6, November/December 2000
Page(s) 671 - 677
DOI: 10.1051/apido:2000151

Apidologie 31 (2000) 671-677

Organ specificity of water-soluble proteins during drone (Apis mellifera L.) ontogenesis

Evgenija Ivanova

Paissij-Hilendarski Universität Plovdiv, Biologische Fakultät, Sektion Genetik. Zar Assenstr. 24, Plovdiv, Bulgarien

(Received 30 October 1999; revised 15 March 2000; accepted 20 July 2000)


Using polyacrylamide gel electrophoresis (PAGE), 368 organ extracts of drones were investigated. The soluble proteins spectra were analyzed in separate organs from the different specimens at various ontogenetic stages (unsealed larvae, sealed larvae, white-eyed pupae, dark-eyed pupae and imago forms). The organs tested (heart, gut, testicle, mucus glands and eyes) were isolated by dissection, rinsed with distilled water, squashed in 0.1 M tris-phosphate buffer at pH 6.7, and left for extraction for 24 h at 4 $^\circ$C. Then the samples were centrifuged for 30 min at 900 g. For electrophoretic separation, a 7.5% polyacrylamide vertical gel (pH 8.9) was used, together with 3.3% concentrating gel at pH 6.7 and 0.05 M tris - 0.2 M glycine electrode buffer, at pH 8.3. The soluble proteins were visualised with Commassie Brilliant Blue R 250. A total of 31 fractions of water-soluble proteins were found in the spectra of analyzed organs - 22 in heart extract, 24 in extract of gut, 21 in extracts of testes, 20 - in extracts of mucus glands and 16 - in eye extracts (Tab. I). An organ and stage specificity in the appearance of soluble proteins during development of Apis mellifera drones could be shown. Organ specific bands were found in the reproductive organs: testis ((A and G) and mucus glands (A), as well as in the intestine (K, L and ZC) and the heart (ZA + ZE) (Figs. 1 and 2).

Keywords: Apis mellifera / drone / ontogenesis / water-soluble proteins

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