Application of solid phase microextraction to evaluate traces of thymol in honeyLara Piasenzottoa, Luisa Graccoa, Lanfranco S. Contea and Stefan Bogdanovb
a Dipartimento di Scienze degli Alimenti Università degli Studi di Udine, Via Marangoni 97, 33100 Udine, Italy
b Schweizerisches Zentrum für Bienenfschung, Liebefeld Schwarzenburgstrasse 155, 3003 Bern, Switzerland
(Received 20 October 2001; revised 7 November 2001; accepted 5 April 2002)
The solid phase microextraction technique was tested for thymol evaluation in honey. Thymol can be present in honey as a residue of treatments against Varroa destructor Honey was sampled from apiaries treated with anti-Varroa products whose active ingredient is thymol. Thymol evaluation was done using the internal standard method; benzophenone and carvacrol were tested as internal standards/ the best results were obtained using benzophenone. The application of an alkaline hydrolysis was important for obtaining quantitative recoveries.
Key words: honey / solid phase microextraction / thymol / Varroa destructor / gas chromatography
Correspondence and reprints: Lanfranco S. Conte
© INRA, EDP Sciences, DIB, AGIB 2002