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Volume 33, Number 6, November-December 2002
Page(s) 545 - 552
Apidologie 33 (2002) 545-552
DOI: 10.1051/apido:2002038

Application of solid phase microextraction to evaluate traces of thymol in honey

Lara Piasenzottoa, Luisa Graccoa, Lanfranco S. Contea and Stefan Bogdanovb

a  Dipartimento di Scienze degli Alimenti Università degli Studi di Udine, Via Marangoni 97, 33100 Udine, Italy
b  Schweizerisches Zentrum für Bienenfschung, Liebefeld Schwarzenburgstrasse 155, 3003 Bern, Switzerland

(Received 20 October 2001; revised 7 November 2001; accepted 5 April 2002)

The solid phase microextraction technique was tested for thymol evaluation in honey. Thymol can be present in honey as a residue of treatments against Varroa destructor Honey was sampled from apiaries treated with anti-Varroa products whose active ingredient is thymol. Thymol evaluation was done using the internal standard method; benzophenone and carvacrol were tested as internal standards/ the best results were obtained using benzophenone. The application of an alkaline hydrolysis was important for obtaining quantitative recoveries.

Key words: honey / solid phase microextraction / thymol / Varroa destructor / gas chromatography

Correspondence and reprints: Lanfranco S. Conte

© INRA, EDP Sciences, DIB, AGIB 2002