Tracking an invasive honey bee pest: mitochondrial DNA variation in North American small hive beetlesJay. D. Evansa, Jeff. S. Pettisa, W. Michael Hoodb and Hachiro Shimanukia
a USDA-ARS Bee Research Lab, Bldg. 476 BARC-E, Beltsville, MD 20705, USA
b Department of Entomology, Clemson University, Clemson, SC 29634, USA
(Received 14 August 2001; revised 15 May 2002; accepted 12 August 2002)
We describe the current and past distributions of North American small hive beetles (Aethina tumida) having two distinct mitochondrial DNA haplotypes. A collection of 539 hive beetles showed irregular distributions of these haplotypes across the southeastern US. Beetles from the first collections made in coastal South Carolina showed haplotype NA1, exclusively. This haplotype is less common in Georgia and was not observed in North Carolina. Later collections from this and other parts of South Carolina appear more similar to those found in other states. The body size of beetles was not correlated with their haplotype, suggesting that differences in haplotype frequency do not reflect selection pressures on covarying differences in the genomes of these beetles. We discuss the implications for inferring the number of separate hive beetle introductions to the US, and for estimating the migration dynamics by hive beetles as they expand their ranges in the New World.
Key words: Apis mellifera / mtDNA / invasive pest / Nitidulidae / Aethina tumida
Correspondence and reprints: Jay. D. Evans
© INRA, EDP Sciences, DIB, AGIB 2003