Molecular characterisation of indigenous Apis mellifera carnica in SloveniaSimona Susnik, Peter Kozmus, Janez Poklukar and Vladimir Meglic
Agricultural Institute of Slovenia, Hacquetova 17, 1000 Ljubljana, Slovenia
(Received 31 October 2002; revised 18 July 2003; accepted 26 March 2004)
Abstract - The genetic structure of Apis mellifera carnica bee from Slovenia, collected from 269 localities of ten Slovenian districts, was assessed by mitochondrial and nuclear DNA analyses. The level of genetic variability within and among districts was low. All of the samples were fixed for one newly found mtDNA haplotype of the C phylogenetic lineage, designated as C2C. A low level of variability was observed for all microsatellite loci, showing a very homogenous structure of the Carniolan bee population. Samples collected in the neighbouring district of Croatia expressed very similar results. On the other hand, high genetic differentiation was observed in comparison with A. m. macedonica population. Present study represents the first attempt to characterise indigenous honeybee populations in Slovenia using molecular methods. Furthermore, it indicates that the Carniolan bee from Slovenia still represents an indigenous gene pool within A. m. carnica.
Key words: A. m. carnica / A. m. macedonica / mtDNA / COI-COII / microsatellite
Corresponding author: Vladimir Meglic firstname.lastname@example.org
© INRA, EDP Sciences, DIB, AGIB 2004