Free access
Volume 31, Number 2, March-April 2000
Taxonomy and Evolutionary biology of the Honeybees
Page(s) 167 - 180
DOI: 10.1051/apido:2000114

Apidologie 31 (2000) 167-180

Molecular confirmation of a fourth lineage in honeybees from the Near East

Pierre Francka - Lionel Garneryb - Michel Solignacb - Jean-Marie Cornueta

aLaboratoire de Modélisation et Biologie Evolutive, URLB-INRA, 488 rue Croix de Lavit, 34090 Montpellier, France
bLaboratoire Population, Génétique et Évolution, CNRS, Gif-sur-Yvette, France


The mitochondrial DNA (mtDNA) from 75 honeybee colonies from the Lebanon was characterized by DraI restriction fragment length polymorphism (RFLP) of the COI-COII intergenic region. The seven observed haplotypes were different enough from all haplotypes already known in Apis mellifera to justify their assignment to a fourth mtDNA lineage. The nucleotide sequence of a 380 base pair (bp) fragment of the NADH2 gene was determined for two haplotypes, which showed a high similarity with two published sequences from A. m. lamarkii and A. m. meda. A microsatellite analysis of a large Lebanese population sample (50 colonies, 8 loci) suggests that Near East populations are also differentiated at the nuclear level from the three previously characterized evolutionary branches of the species A. mellifera.

Keywords: Apis mellifera / mtDNA / microsatellite / evolutionary history / Near East

Correspondence and reprints: Jean-Marie Cornuet

Copyright INRA/DIB/AGIB/EDP Sciences

What is OpenURL?

The OpenURL standard is a protocol for transmission of metadata describing the resource that you wish to access.

An OpenURL link contains article metadata and directs it to the OpenURL server of your choice. The OpenURL server can provide access to the resource and also offer complementary services (specific search engine, export of references...). The OpenURL link can be generated by different means.

  • If your librarian has set up your subscription with an OpenURL resolver, OpenURL links appear automatically on the abstract pages.
  • You can define your own OpenURL resolver with your EDPS Account.
    In this case your choice will be given priority over that of your library.
  • You can use an add-on for your browser (Firefox or I.E.) to display OpenURL links on a page (see You should disable this module if you wish to use the OpenURL server that you or your library have defined.