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Table I
RDA adapters and quantitative PCR primer sequences and their respective target genes.
| RDA-adapters | Symbol | Sequence | |||
|---|---|---|---|---|---|
| R12 | 5’- GATCTGCGGTGA -3’ | ||||
| R24 | 5’- AGCACTCTCCAGCCTCTCACCGCA -3’ | ||||
| J12 | 5’- GATCTGTTCATG -3’ | ||||
| J24 | 5’- ACCGACGTCGACTATCCATGAACA -3’ | ||||
| N12 | 5’- GATCTTCCCTCG -3’ | ||||
| N24 | 5’- AGGCAACTGTGCTATCCGAGGGAA -3’ | ||||
| Target genes – real-time PCR | |||||
| acid phosphatase 1 | Acph-1FAcph-1R | 5’- TCACCAAGCATATGTTAGACG -3’5’- CAGGAACGTGAGGATAATAAAG -3’ | |||
| CG14516 | CG14516FCG14516R | 5’- CAGCCATAAGGGAAGGAAAT -3’5’- GCGCGATTCTGAGATATGAG -3’ | |||
| atlastin | atlFatlR | 5’- CCATTACGTGGATTTTCGTG -3’5’- GGATTGACTATCGAAAGCAC -3’ | |||
| bitesize | btszFbtszR | 5’- GGAGAGATACCGCGTTTGTG -3’5’- GACCAATCTGCTGCCTATGTG -3’ | |||
| ypsilon schachtel | ypsFypsR | 5’- CAATCCGAGAAAGGCTGTAC -3’5’- GTCGCTTATCGGCAGCATAA -3’ | |||
| CG11791 | CG11791FCG11791R | 5’- GACCAGGATACACTTGTACAC -3’5’- GATCCTTTAATTCAGGAGGAA -3’ | |||
| 14-3-3 ε | 14-3-3 εF14-3-3 εR | 5’- CCTGATAGAGCATGTCGTCT -3’5’- CCTTGCATGTCTGACGTCC -3’ | |||
| stretchin-mlck | Strn-MlckFStrn-MlckR | 5’- TCGGATCCAAAGAGGCATAAC -3’5’- GATTAATAGCCTCGGGTCCTC -3’ | |||
| kayak | kayFkayR | 5’- CGACAGATCCGCAGAGAAAG -3’5’- CCTGTTGCAGCTGTTGTATC -3’ | |||
| polyprotein deformed wing virus | DWVFDWVR | 5’- CGACATAGATGACTAGGCGC -3’5’- CCTGGATTAGATTCCGATGT -3’ | |||
| GB17584 | GB17584FGB17584R | 5’- GAATACAGCGGTGAAGGAATC -3’5’- GTTTCTTGCCAGGTTGATGC -3’ | |||
| GB18642 | GB18642FGB18642R | 5’- CGAAGGCAAACGAGGAAGAG -3’5’- CGATGATGTGGCCGAGACTA -3’ | |||
| rp49 | RP49-FRP49-R | 5’-CGTCATATGTTGCCAACTGGT-3’5’-TTGAGCACGTTCAACAATGG-3’ | |||