Free Access
Table II
ESTs sequenced from two Representational Difference Analysis (RDA) libraries of mucus gland mRNA of newly emerged drones. These ESTs were mapped to the honey bee genome and compared to non-redundant (nr) databases. Shown are the genomic location (Group in the Amel 3.0 assembly) and GLEAN3-predicted CDS (GB - gene number in Official Gene Set 1; The Honeybee Genome Sequencing Consortium, 2006), hit localization of ESTs (to CDS or putatve UTR) and the number of ESTs mapping to each site, the respective fly or human orthologs retrieved by BLASTP searches in GenBank, followed by the similarity index (e-value) and corresponding Gene Ontology terms obtained from Flybase (http://flybase.bio.indiana.edu/).
| Group | GB | Localization | ESTs | Ortholog | e-value | Biological | |
|---|---|---|---|---|---|---|---|
| # | (fly or human) | process | |||||
| GroupUn.1897 | GB12546 | CDS | 28 | acid phosphatase | 2e-51 | phosphate metabolism | |
| Group10.14 | GB17584 | CDS | 11 | predictedhypothetical protein | 5e-41 | – | |
| GroupUn.3 | GB11965 | CDS | 06 | myosin heavychain | 0.0 | cytokinesis; mitosis; myofibril assembly; striated muscle contraction | |
| Group7.11 | GB14689 | CDS | 03 | peptidase | 0.0 | proteolysis | |
| GroupUn.1159 | GB14853 | CDS | 02 | atlastin | 0.0 | immune response | |
| Group11.2 | GB15345 | CDS | 02 | CG2989 | 1e-171 | cell-cell signaling; chitin and polysaccharide metabolism; signal transduction | |
| Group9.1 | GB13141 | 5’UTR - 53 bp and 214 bp | 02 | shaggy | 1e-13 | intracellular signaling cascade; Notch and Wnt receptor signaling pathway; negative regulation of smoothened signaling pathway; oogenesis; protein amino acid phosphorylation; regulation of proteolysis and transcription | |
| Group11.26 | GB13617 | CDS | 01 | bitesize | 3e-122 | positive regulation of body size; positive regulation of cell size; synaptic vesicle transport | |
| GroupUn.460 | GB12819 | CDS | 01 | CG14439 | 3e-176 | transport | |
| Group7.11 | GB17753 | CDS | 01 | aminopeptidase | 5e-149 | proteolysis | |
| Group2.1 | GB17562 | CDS | 01 | CG8801 | 0.0 | cell proliferation | |
| Group16.5 | GB11237 | CDS | 01 | suppressor of variegation 3-9 | 0.0 | DNA methylation; chromatin assembly or disassembly; chromatin silencing; establishment and/or maintenance of chromatin architecture; gene silencing; histone H3-K9 methylation; histone methylation; histone modification; oogenesis; translational initiation | |
| Group16.10 | GB18446 | CDS | 01 | ypsilon schachtel | 1e-22 | oogenesis; regulation of transcription from RNA polymerase II promoter | |
| Group13.4 | GB19169 | CDS | 01 | CG15105 | 6e-152 | cell proliferation; nucleic acid metabolism; protein ubiquitination; regulation of transcription from RNA polymerase II promoter; transport | |
| Group1.25 | GB11183 | 3’UTR- 253bp | 01 | basigin | 2e-24 | spermatid development | |
| GroupUn.89 | GB16541 | CDS | 01 | CG11791 | 5e-70 | – | |
| Group2.5 | GB18642 | CDS | 01 | predictedhypothetical protein | 0.0 | – | |
| GroupUn.447 | GB12575 | CDS | 01 | fau | 7e-10 | – | |
| Group6.27 | GB16247 | CDS | 01 | KIAA1345 | 8e-24 | – | |
| Group8.18 | GB17780 | CDS | 01 | CG8483 | 5e-38 | defense response |