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Table I
Results from a repeated measures analysis of variance of average relative amounts of volatile compounds collected from live honey bee queens at three different developmental states (virgins < 48 h old, virgins 7–8 days old, and mated egg-laying queens). Each queen sampled was either open-mated (n=10) or instrumentally inseminated (n = 9 for virgin queens and n = 8 for egg-laying queens) [queen type]. Volatiles were analyzed using gas chromatography and mass spectroscopy. Compounds were either identified or characterized as peaks on a gas chromatogram. For all queen type data, d.f. = 1; for all queen state data, d.f. = 2; type × state, d.f. = 2.
| Peak | Queen Type | Queen State | Type × State | ||||
|---|---|---|---|---|---|---|---|
| F | P | F | P | F | P | ||
| 1 | 1.62 | 0.212 | 3.7 | 0.035 | 0.43 | 0.65 | |
| 4 (n-octanal) | 1.25 | 0.271 | 22.1 | < 0.0001 | 1.21 | 0.31 | |
| 5 (E- β-ocimene) | 0.52 | 0.730 | 32.0 | < 0.0001 | 0.55 | 0.58 | |
| 6 | 2.58 | 0.12 | 35.4 | < 0.0001 | 2.88 | 0.07 | |
| 7 (2-phenylethanol) | 0.22 | 0.64 | 5.8 | 0.006 | 0.91 | 0.41 | |
| 8 (long chain hydrocarbon) | 0.20 | 0.66 | 12.3 | < 0.0001 | 0.04 | 0.96 | |
| 9 (n-decanal) | 0.06 | 0.80 | 23.7 | < 0.0001 | 0.03 | 0.97 | |
| 12 (long chain hydrocarbon) | 1.40 | 0.24 | 8.0 | 0.001 | 0.81 | 0.45 | |