Free access
Volume 31, Number 1, January-Febuary 2000
Page(s) 17 - 23
DOI: 10.1051/apido:2000101

Apidologie 31 (2000) 17-23

Nucleotide sequence variations in Kashmir bee virus isolated from Apis mellifera L. and Varroa jacobsoni Oud.

Akey C.F. Hung ${^{\rm a}}$, Christine Y.S. Peng ${^{\rm b}}$, H. Shimanuki ${^{\rm a}}$

${^{\rm a}}$Bee Research Laboratory, USDA-ARS, Beltsville, MD 20705, USA
${^{\rm b}}$Department of Entomology, University of California, Davis, CA 95616, USA

(Received 14 June 1999; accepted 19 August 1999)


The 374 bp amplicon of 20 Kashmir bee virus (KBV) isolates from 14 bee and 6 mite samples from California was sequenced. The sequence data and those of homologous KBV amplicons of one bee each from Australia, Canada, Maine, and Maryland were analyzed. Pairwise comparisons of nucleotide sequences show the mean distance between Californian isolates varying from 0% to 3.2%. The mean distance between Californian isolates and the two U.S. or the Canadian isolates are equal or less than 3.2%. The Australian isolate is different from all North American isolates with mean distances of 11.5 to 12.3%. This 374 bp sequence contains a single open reading frame, the 124 amino acid sequence matched closely with the sequences of many virus polyproteins.

Kasmir bee virus / direct RT-PCR / pairwise comparison / phylogenetic tree

Correspondence and reprints: Akey C.F. Hung

Copyright INRA/DIB/AGIB/EDP Sciences