Free Access
Issue
Apidologie
Volume 34, Number 6, November-December 2003
Page(s) 597 - 602
DOI https://doi.org/10.1051/apido:2003055
Apidologie 34 (2003) 597-602
DOI: 10.1051/apido:2003055

A method to feed individual bees (Hymenoptera: Apiformes) known amounts of pesticides

Edith Ladurnera, Jordi Boschb, Stefano Mainia and William P. Kempc

a  Dipartimento di Scienze e Tecnologie Agroambientali, Università di Bologna, 40126 Bologna, Italy
b  Biology Department, Utah State University, Logan, UT 84322-5310, USA
c  USDA-ARS, Bee Biology & Systematics Laboratory, Logan, UT 84322-5310, USA

(Received 10 October 2002; revised 1 February 2003; accepted 16 May 2003)

Abstract
We devised a simple method ("flower") to feed bees individually, and compared it with two other methods commonly used ("film canister" and "glass vial"). We tested the three methods on two solitary species, Osmia lignaria and Megachile rotundata, and one social species, Apis mellifera, under four different light regimes (natural, artificial, plant growth and darkness). The flower method was the most effective for all three bee species: 90-95% of the bees fed under natural light, 80-95% under artificial light, 75-100% under plant growth light, and 45-70% in darkness. Percent success was 0-50% with the film canister method, and 0-60% with the glass vial method. The flower method may allow more comprehensive future evaluation of pesticide effects on bees.


Key words: oral toxicity test / individual feeding / Osmia lignaria / Apis mellifera / Megachile rotundata

Correspondence and reprints: Jordi Bosch Nomada@biology.usu.edu

© INRA, EDP Sciences, DIB, AGIB 2003

Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.

Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.

Initial download of the metrics may take a while.