Free access
Volume 37, Number 5, September-October 2006
Page(s) 507 - 516
Published online 24 June 2006
Apidologie 37 (2006) 507-516
DOI: 10.1051/apido:2006030

In situ localization of heat-shock proteins and cell death labelling in the salivary gland of acaricide-treated honeybee larvae

Elaine C.M. Silva-Zacarina, Ales Gregorcb and Regina L.M. Silva de Moraesc

a  Federal University of São Carlos, UFSCar, Sorocaba, SP, Brasil
b  Agricultural Institute of Slovenia, Hacquetova 17, 1001 Ljubljana, Slovenia
c  Departamento de Biologia, I.B., Universidade Estadual Paulista (UNESP), Av. 24 A, n. 960, CEP: 13506-700, PO Box 199, Rio Claro/SP, Brazil

(Received 18 August 2005 - revised 19 November 2005 - accepted 22 November 2005 - published online 24 June 2006)

Abstract - The effects of the acaricides, rotenone and oxalic acid (OA) on salivary glands of honeybee larvae were evaluated. Immunohistochemical methods were used to detect cell death and heat-shock protein (HSP70 and 90) localizations. Heat-shock proteins (HSP70 and 90) were localized in the cytoplasm and/or the nuclei of secretory gland cells, both under stress and in normal conditions. In rotenone-treated larvae, there were no changes in the normal level of cell death and also there were no morphological alterations in the secretory cells. In the larvae treated with oxalic acid, the salivary gland showed varying degrees of morphological cellular alteration and an increase in the cell death level. The present data suggest that stress-induced HSP70 might have an antiapoptotic effect while the stress-induced HSP90 might have a chaperone function in the larval salivary glands.

Key words: Apis mellifera / silk gland / cellular stress / immunohistochemistry / rotenone / oxalic acid

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© INRA, DIB-AGIB, EDP Sciences 2006