Free Access
| Issue |
Apidologie
Volume 22, Number 6, 1991
Evolution and genetics
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|---|---|---|
| Page(s) | 611 - 619 | |
| DOI | https://doi.org/10.1051/apido:19910604 | |
Apidologie 22 (1991) 611-619
DOI: 10.1051/apido:19910604
a Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Genética, 14049 Ribeirão Preto, São Paulo, SP, Brazil
b Universidade de Sâo Paulo, Instituto de Biociências, Departamento de Biologia, Caixa Postal 11461, 05499 São Paulo, SP, Brazil
Key words: Africanized honey bee / mitochondrial gene / gene mapping / restriction endonuclease / molecular hybridization
DOI: 10.1051/apido:19910604
Location of genes in Apis mellifera scutellata-derived mitochondrial DNA of Africanized honey bees
M.C. Ariasa and F.G. Nobregaba Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Genética, 14049 Ribeirão Preto, São Paulo, SP, Brazil
b Universidade de Sâo Paulo, Instituto de Biociências, Departamento de Biologia, Caixa Postal 11461, 05499 São Paulo, SP, Brazil
Abstract - The mitochondrial DNA of Africanized honey bees having a mtDNA haplotype known from the African subspecies Apis mellifera scutellata, was analysed for the location of 6 mitochondrial genes by hybridization to mitochondrial gene probes from Saccharomyces cerevisiae. Genes for the small and large ribosomal RNAs, cytochrome oxidase subunits I, II and III and apocytochrome b were positioned relative to the mitochondrial DNA fragments generated with 6 different restriction endonucleases utilized singly or in combination. Results show that these genes are in the same relative positions as Drosophila genes.
Key words: Africanized honey bee / mitochondrial gene / gene mapping / restriction endonuclease / molecular hybridization