Free Access
| Issue |
Apidologie
Volume 31, Number 4, July-August 2000
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|---|---|---|
| Page(s) | 543 - 554 | |
| DOI | https://doi.org/10.1051/apido:2000145 | |
DOI: 10.1051/apido:2000145
Apidologie 31 (2000) 543-554
Interactions among Varroa jacobsoni mites, acute paralysis virus, and Paenibacillus larvae larvae and their influence on mortality of larval honeybees in vitro
Camilla J. Brødsgaarda - Wolfgang Ritterb - Henrik Hansena - Henrik F. Brødsgaarda
aDanish Institute of Agricultural Sciences, Research Group Entomology,
Research Centre Flakkebjerg, 4200 Slagelse, Denmark
bChemische und Veterinäruntersuchungsamt Freiburg, Tierhygiene, Fachgebiet : Bienen,
79018 Freiburg, Germany
(
Abstract:
The mortality of honey bee larvae and pupae reared in vitro caused by various combinations
of oral inoculation with Paenibacillus larvae larvae spores, acute paralysis virus
(APV), and infestation with Varroa jacobsoni was studied. The effect of the mite
itself and the mite acting as a vector of APV on the mortality of larvae and pupae was
investigated. Mortality caused by P. l. larvae ranged from 25 to 55% depending on
spore dose. Oral inoculation with APV caused 9% mortality, which was not additive to the
mortality caused by P. l. larvae. P. l. larvae did not induce the activation of
APV infection. The mortality caused by V. jacobsoni itself was 25%, and by mites
transmitting APV, 55%. Neither the mites themselves or the mites transmitting APV had an
additive effect on mortality caused by P. l. larvae. The study suggests that APV
transmitted by mites is the most significant cause of mortality of the treatment
combinations tested. The results do not suggest that the mite itself or the mites
transmitting APV act as a stress factor provoking clinical symptoms of American foulbrood
(AFB) in individual larvae in the in vitro rearing system.
Keywords:
Varroa jacobsoni / Paenibacillus larvae larvae / American
foulbrood / acute paralysis virus / APV / honey bee / Apis mellifera
Correspondence and reprints: Camilla J. Brodsgaard
E-mail: camilla.brodsgaard@agrsci.dk
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