Free Access
| Issue |
Apidologie
Volume 31, Number 4, July-August 2000
|
|
|---|---|---|
| Page(s) | 517 - 524 | |
| DOI | https://doi.org/10.1051/apido:2000103 | |
DOI: 10.1051/apido:2000103
Apidologie 31 (2000) 517-524
Analysis of oxytetracycline in extender patties
Jan Kochansky
USDA ARS Bee Research Laboratory, Building 476, 10300 Baltimore Ave., Beltsville,
MD 20705-2350, USA
(
Abstract:
Antibiotic extender patties, consisting of sugar, vegetable shortening, and oxytetracycline
(OTC, usually as the hydrochloride), are used for treatment/prevention of foulbrood in honey
bees. An analytical method was developed to determine the concentration in the patties and
to allow a study of deterioration over time. Trituration of a sample with EDTA-treated
C18 silica gel, removal of the shortening with isooctane, and elution of the OTC with
methanol/acetonitrile gave a solution that could be analyzed by HPLC. Different
concentrations and total amounts of OTC have been recommended by different authors. Not all
newly-procured samples contained the same level of OTC, and the concentration decreased
about 4% per year with storage at room temperature.
Keywords:
oxytetracycline / solid-phase extraction / HPLC / American foulbrood / European
Correspondence and reprints: Jan Kochansky
E-mail: jkochans@asrr.arsusda.gov
Copyright INRA/DIB/AGIB/EDP Sciences