Free Access
| Issue |
Apidologie
Volume 31, Number 5, September-October 2000
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|
|---|---|---|
| Page(s) | 567 - 577 | |
| DOI | https://doi.org/10.1051/apido:2000147 | |
DOI: 10.1051/apido:2000147
Apidologie 31 (2000) 567-577
Detection of chronic honey bee (Apis mellifera L.) paralysis virus infection: application to a field survey
Magali Ribière - Jean-Paul Faucon, Michel Pépin
AFSSA Sophia Antipolis, Unité Abeille, BP 111, 06902 Sophia Antipolis, France
(
Abstract:
Chronic paralysis of honey bees is a viral disease caused by chronic paralysis virus
(CPV). As its clinical signs are similar to those observed in intoxications or during
intense work, it requires sensitive and specific diagnostic tests to identify the
infection in colonies. In this report, a rabbit polyclonal antiserum against CPV was
obtained after purification of the virus in a Renografin gradient. The viral polypeptide
composition was analysed by SDS-PAGE and Western blotting. Four viral-associated
polypeptides of molecular weights 75 kDa, 50 kDa, 30 kDa and 20 kDa were identified.
Western blotting and an agar gel immunodiffusion test were used for the diagnosis of
CPV during a field survey of the prevalence of CPV infection. The two tests demonstrated
the presence of the virus in several colonies in the south-east of France.
Keywords:
chronic paralysis virus (CPV) / polypeptide composition / diagnosis / epidemiology
Correspondence and reprints: Magali Ribière
e-mail: m.ribiere@sophia.afssa.fr
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