Volume 32, Number 5, September-October 2001
|Page(s)||407 - 416|
Apidologie 32 (2001) 407-416
Apis dorsata drone flights, collection of semen from everted endophalli and instrumental insemination of queensJerzy Woykea, Jerzy Wildeb and Maria Wildec
a Agricultural University - SGGW, Bee Division, 166 Nowoursynowska 02-787 Warsaw, Poland
b WM University, Apiculture Division, Olsztyn, Poland
c Dabur Apicultural Center, Jugedi, Chitwan, Nepal
(Received 30 November 2000; revised 28 April 2001; accepted 18 May 2001)
We observed drone flights of 16 colonies of Apis dorsata in Chitwan, Nepal. At the end of February drone flights occurred between 18:15 and 18:35 h. By April, as day length increased, drones flew gradually later. Within 2 months the start of drone flights was delayed by 42 min. High correlation (r = 0.99) was found between the sunset time and the start of drone flights. After the thorax or abdomen of drones were squeezed, seven stages of endophallus eversion occurred. Semen appeared as a small drop at the ventral side of completely everted cervix. We collected 8 mm3 of semen from 41 drones. Thus, one drone produced on average 0.2 mm3 of semen. Three Apis mellifera queens were inseminated with 2-3 mm3 of A. dorsata semen. All queens started to lay eggs. Larvae hatched from 3 were found. We speculate that embryos in rest of the eggs did not develop due to genetic incompatibility.
Key words: drones / drone flight time / eversion of endophallus / semen / instrumental insemination / Apis dorsata
Correspondence and reprints: Jerzy Woyke
© INRA, EDP Sciences, DIB, AGIB 2001
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.