Free Access
Issue |
Apidologie
Volume 34, Number 2, March-April 2003
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Page(s) | 181 - 188 | |
DOI | https://doi.org/10.1051/apido:2003001 |
Apidologie 34 (2003) 181-188
DOI: 10.1051/apido:2003001
a Departamento de Química Analítica, Facultad de Ciencias, Universidad de Valladolid, 47005 Valladolid, Spain
b Centro Apícola Regional Junta de Comunidades de Castilla La Mancha, 19180 Marchamalo, Guadalajara, Spain
c Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, Madrid, Spain
(Received 11 January 2002; revised 7 August 2002; accepted 7 October 2002)
Key words: ion exclusion chromatography / oxalic acid / organic acids / honey / honeybee
Correspondence and reprints: María Jesús Nozal
e-mail: mjdnozal@qa.uva.es
© INRA, EDP Sciences, DIB, AGIB 2003
DOI: 10.1051/apido:2003001
Determination of oxalic acid and other organic acids in honey and in some anatomic structures of bees
María Jesús Nozala, José Luis Bernala, Luis Antonio Gómeza, Mariano Higesb and Aranzazu Meanaca Departamento de Química Analítica, Facultad de Ciencias, Universidad de Valladolid, 47005 Valladolid, Spain
b Centro Apícola Regional Junta de Comunidades de Castilla La Mancha, 19180 Marchamalo, Guadalajara, Spain
c Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, Madrid, Spain
(Received 11 January 2002; revised 7 August 2002; accepted 7 October 2002)
Abstract
The use of two ion-exclusion columns coupled in series allows the liquid chromatographic
determination of the oxalic acid content in honey and in some anatomic structures of bees,
and avoids interference from other components of the matrix. To obtain a profile of organic
acids, it is advisable to couple of four columns in series. The oxalic acid concentration
in the rectum, Malpighian tubules, digestive tract and haemolymph is strongly influenced
by the application mode (topical or oral). Results obtained demonstrate that in topical
administration oxalic acid penetrates through the keratin.
Key words: ion exclusion chromatography / oxalic acid / organic acids / honey / honeybee
Correspondence and reprints: María Jesús Nozal
e-mail: mjdnozal@qa.uva.es
© INRA, EDP Sciences, DIB, AGIB 2003