Free Access
Volume 36, Number 1, January-March 2005
Page(s) 113 - 118
Published online 16 March 2005
Apidologie 36 (2005) 113-118
DOI: 10.1051/apido:2004075

Non-radioactive in-situ hybridisation to honeybee embryos and ovaries

Peter Osborne and Peter K. Dearden

Laboratory for Development and Evolution, Biochemistry Department, University of Otago, Dunedin, New Zealand

(Received 5 July 2004 - Revised 23 August 2004 - Accepted 26 August 2004; Published online: 16 March 2005)

Abstract - To study the function and expression of genes identified in the honeybee genome sequence, techniques for assaying gene expression need to be developed. We present a robust method for preparing honeybee embryos and ovaries for in-situ hybridisation. This protocol should prove useful for researchers wishing to use the genome sequence to study honeybee biology.

Key words: Apis mellifera / embryo / ovary / gene expression / in-situ hybridisation

Corresponding author: Peter K. Dearden

© INRA, DIB-AGIB, EDP Sciences 2005