Free Access
| Issue |
Apidologie
Volume 36, Number 1, January-March 2005
|
|
|---|---|---|
| Page(s) | 113 - 118 | |
| DOI | https://doi.org/10.1051/apido:2004075 | |
| Published online | 16 March 2005 | |
Apidologie 36 (2005) 113-118
DOI: 10.1051/apido:2004075
Laboratory for Development and Evolution, Biochemistry Department, University of Otago, Dunedin, New Zealand
(Received 5 July 2004 - Revised 23 August 2004 - Accepted 26 August 2004; Published online: 16 March 2005)
Key words: Apis mellifera / embryo / ovary / gene expression / in-situ hybridisation
Corresponding author: Peter K. Dearden peter.dearden@stonebow.otago.ac.nz
© INRA, DIB-AGIB, EDP Sciences 2005
DOI: 10.1051/apido:2004075
Non-radioactive in-situ hybridisation to honeybee embryos and ovaries
Peter Osborne and Peter K. DeardenLaboratory for Development and Evolution, Biochemistry Department, University of Otago, Dunedin, New Zealand
(Received 5 July 2004 - Revised 23 August 2004 - Accepted 26 August 2004; Published online: 16 March 2005)
Abstract - To study the function and expression of genes identified in the honeybee genome sequence, techniques for assaying gene expression need to be developed. We present a robust method for preparing honeybee embryos and ovaries for in-situ hybridisation. This protocol should prove useful for researchers wishing to use the genome sequence to study honeybee biology.
Key words: Apis mellifera / embryo / ovary / gene expression / in-situ hybridisation
Corresponding author: Peter K. Dearden peter.dearden@stonebow.otago.ac.nz
© INRA, DIB-AGIB, EDP Sciences 2005