Issue |
Apidologie
Volume 38, Number 4, July-August 2007
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Page(s) | 315 - 322 | |
DOI | https://doi.org/10.1051/apido:2007017 | |
Published online | 10 October 2007 |
DOI: 10.1051/apido:2007017
Study of the depletion of tylosin residues in honey extracted from treated honeybee (Apis mellifera) colonies and the effect of the shook swarm procedure
Stuart J. Adamsa, Katharina Heinricha, Mike Hetmanskia, Richard J. Fussella, Selwyn Wilkinsb, Helen M. Thompsonb and Matthew Sharmanaa Food Safety Group, Central Science Laboratory, Sand Hutton, York, YO41 1LZ, UK
b National Bee Unit, Central Science Laboratory, Sand Hutton, York, YO41 1LZ, UK
(Received 5 July 2006 - Revised 7 December 2006 - Accepted 22 December 2006 - Published online 10 October 2007)
Abstract - Bee colonies were dosed with tylosin tartrate 1.1 g per
hive (single dose in sucrose solution) and samples of honey were then
collected at intervals over a 20-week period. The samples were analysed for
tylosin A and desmycosin (tylosin B) using LC-MS/MS. The mean concentration
of tylosin A in the honey (pooled results) 3 days after dosing was 17 g/g, declining to
0.9
g/g after 140 days. The mean concentration of desmycosin was 2.3
g/g, 3 days after dosing declining to 1.1
g/g after 140 days.
The shook swarm procedure was investigated and resulted in a tylosin A
concentration in brood honey of 10
g/g, 3 days after dosing declining
to 0.02
g/g, 140 days after dosing. A corresponding decrease in the
mean concentrations of desmycosin in brood honey, 1.1
g/g, 3 days
after dosing to 0.03
g/g, 140 days after dosing also was observed.
Tylosin A depletes to desmycosin in honey and can still be detected 238 days
after dosing. Thus a more accurate residue definition is the sum of tylosin
A and desmycosin.
Key words: Tylosin / desmycosin / honey / veterinary drug / residues / apiculture
© INRA, DIB-AGIB, EDP Sciences 2007