An oligonucleotide primer set for PCR amplification of the complete honey bee mitochondrial genome

Maria Cristina Arias; Daniela Silvestre; Flávio de Oliveira Francisco; Ricardo Weinlich; Walter Steven Sheppard

doi:doi:10.1051/apido:2008024

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Volume 39 / No 4 (July-August 2008)

Apidologie, 39 4 (2008) 475-480

Abstract

Free Access

Issue		Apidologie Volume 39, Number 4, July-August 2008


Page(s)		475 - 480
DOI		https://doi.org/10.1051/apido:2008024
Published online		25 June 2008

Apidologie 39 (2008) 475-480
DOI: 10.1051/apido:2008024

An oligonucleotide primer set for PCR amplification of the complete honey bee mitochondrial genome

Maria Cristina Arias¹, Daniela Silvestre¹, Flávio de Oliveira Francisco¹, Ricardo Weinlich¹ and Walter Steven Sheppard²

¹ Departamento de Genética e Biologia Evolutiva, Instituto de Biociências,Universidade de São Paulo, São Paulo, SP, 05508-090, Brazil
² Department of Entomology, Washington State University, Pullman, WA, 99164-6382, USA

Received 4 December 2007 - Revised 5 March 2008 - Accepted 25 March 2008 - Published online 25 June 2008

Abstract - Mitochondrial DNA markers have been widely used to address population and evolutionary questions in the honey bee Apis mellifera. Most of the polymorphic markers are restricted to few mitochondrial regions. Here we describe a set of 24 oligonucleotides that allow PCR amplification of the entire mitochondrial genome of the honey bee A. mellifera in 12 amplicons. These fragments have important applications for the study of mitochondrial genes in different subspecies of A. mellifera and as heterospecific probes to characterize mitochondrial genomes in other bee species.