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Table II

Mean time to death (LT50) of third instar honey bee (Apis mellifera) larvae fed with two different doses of Ascosphaera apis spores. The larvae originated from three different honey bee subspecies, A. m. carnica, A. m. ligustica and A. m. mellifera.

Dose of 1000 spores Dose of 10000 spores
Subspecies na LT50± SE na LT50± SE
(days p.i.) (days p.i.)
A. m. carnica
 Colony 1 104 6.57± 0.21 71 4.96± .025
 Colony 2 127 6.65± 0.21 63 5.24± 0.28
 Colony 3 120 6.43± 0.17 62 4.61± .020
 Colony 4 72 6.06± 0.2 51 5.20± 0.2
 All 5.00± 0.12
A. m. ligustica
 Colony 1 197 6.79± 0.17 109 5.83± 0.23
 Colony 2 115 6.97± 0.21 79 5.82± 0.24
 Colony 3 203 6.89± 0.16 116 6.24± 0.22
 All 5.98± 0.13
A. m. mellifera
 Colony 1 90 6.58± 0.21 45 4.96± 0.25
 Colony 2 154 6.65± 0.21 80 5.24± 0.28
 Colony 3 119 6.43± 0.17 83 4.61± 0.20
 Colony 4 142 6.06± 0.20 75 5.20± 0.23
 All 5.26± 0.11
a

Total number of larvae tested from each colony.