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Table I
Characterization of the microsatellite loci developed for Melipona mondury. GenBank accession numbers are given below locus names.
| Locus | Repeat | Size | Ta | Primer sequence (5’-3’) | A | H |
|---|---|---|---|---|---|---|
| (bp) | (°C) | |||||
| Mmo03(EU735180) | (TAAA)3 | 133 | 50° | F: GGTGAAAAGGGGATGAAGR: ACTGCGTAAAACGAAGAC | 1 | 0.0 |
| Mmo08(EU735181) | (CTTT)2CT(CTTT)3 | 104 | 59° | F: CGAGCTGAAAGGGCGTTR: TCCTCTCTTTTGGTGGTC | 2 | 0.38 |
| Mmo10(EU735182) | (GAA)5 | 106 | 60.5° | F: AGAGAGACGAAGCGAAGR: ATGACAACCGATCACGA | 2 | 0.32 |
| Mmo11(EU735183) | (AG)3GG(AG)6 | 116 | 57° | F: AGAGAGAGACAGAGAGAAR: CGACCCATATTTTCCGAG | 2 | 0.32 |
| Mmo15(EU735184) | (GAAC)5 | 100 | 54° | F: GCAAAATGAGCAAACGGACAR: GAGAGAGAGGGAGGTTC | 2 | 0.49 |
| Mmo19(EU735185) | (GT)17 | 107 | 62° | F: AGCGATTTAACTGAACGATR: TATTCCTCTCTTCCTACAC | 6 | 0.67 |
| Mmo20(EU735186) | (TCG)6 | 121 | 60.5° | F: GAGGGCGACGATTACGACR: AACGGCATTGTGTAAACAGA | 2 | 0.26 |
| Mmo21(EU735187) | (GA)5 | 168 | 57° | F: AACGTGGAACAGTCGGACR: AGCGATTCGTTCAATTCG | 5 | 0.77 |
| Mmo22(EU735188) | (AG)16 | 191 | 61.5° | F: TGTAATCCTAACCCGATTR: CTTTGCAGTTTTCACACG | 5 | 0.79 |
| Mmo24(EU735189) | (AG)4(AG)3(GAAAA)2 | 180 | 52° | F: TCAGCGATTAATGAACACR: TGGAAATGTTTTACCTGT | 3 | 0.10 |