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Table I
Primer sets of major royal jelly protein (mrjp) genes for real-time quantitative reverse transcriptase polymerase chain reaction.
| Gene | Accession | Primer set | Fragment | Primer |
|---|---|---|---|---|
| No. | length (bp) | concentration (nM) | ||
| mrjp1 | AF000633 | Forward: 5’-AACGCGAATGTAAACGAATTGA-3’ | 76 | 100 |
| Reverse: 5’-AAATTTTGAAAGGTGTTCGA-3’ | ||||
| mrjp2 | AF000632 | Forward: 5’-GTAACAGAATGCAGAAAATAGTAAATGATG-3’ | 127 | 150 |
| Reverse: 5’-GTTATCATTCTGATTGTTATTTACGCAATG-3’ | ||||
| mrjp3 | Z26318 | Forward: 5’-TTCAACGACGTAAATTTCCGAAT-3’ | 72 | 75 |
| Reverse: 5’-TCTTCCGCAACGAGTGTTTCT-3’ | ||||
| mrjp4 | Z26319 | Forward: 5’-CTCGAACACGTGAACTTCCAAA-3’ | 68 | 75 |
| Reverse: 5’-GCGCAACGACTATTCCGTATC-3’ | ||||
| mrjp5 | AF004842 | Forward: 5’-TGGGTGCAAATGTAAACGATTT-3’ | 145 | 75 |
| Reverse: 5’-GGTTTATATCGAGAAAAGACTGATTAACAG-3’ | ||||
| mrjp6 | AY313893 | Forward: 5’-GTAACAGAATGCAGAAAATAGTAAACAATG-3’ | 112 | 300 |
| Reverse: 5’-GACTTTGCACAACGAGTGTTTTTTAT-3’ | ||||
| mrjp7 | BK001420 | Forward: 5’-GAAAACACTCGTTGCACAAATTTT-3’ | 85 | 300 |
| Reverse: 5’-CGTCTATTGTGATTCTTATGCTGTCA-3’ | ||||
| mrjp8 | AY398690 | Forward: 5’-CGCGAATGTGAACGACTTGA-3’ | 91 | 300 |
| Reverse: 5’-CAGATTATTGGCTAAGTTGATTTATATCG-3’ | ||||
| mrjp9 | DQ000307 | Forward: 5’-TGCGCCTGTAAATCAATTAATAAGG-3’ | 82 | 300 |
| Reverse: 5’-GGAAGATCAAAGGAAAATTGAGAAAA-3’ |