Free Access
Volume 26, Number 6, 1995
Page(s) 487 - 493
Apidologie 26 (1995) 487-493
DOI: 10.1051/apido:19950605

The insemination of queen honeybees with diluted semen

W. Skowroneka, C. Kruka and K. Locb

a  Bee Department, Institute of Pomology and Floriculture, 24-100 Pulawy, Poland
b  Animal Breeding Station, Zelków, 08-110 Siedlce, Poland

Abstract - The aim of this study was to assess the possibility of queen honeybee insemination with a semen-diluent mixture and to evaluate the rate of insemination and the accuracy of stirring. Queen honeybees inseminated twice with a Hyes solution/semen mixture (1:1), given every other day at 8 μl per dose, stored as many spermatozoa in their spermatheca as those inseminated once by a classical technique. The onset and percentage of oviposition were similar to those observed in other procedures. The diluted mixture showed complete mixing of the semen collected from several drones.

Key words: queen honeybee / instrumental insemination / diluted semen