Free Access
Issue |
Apidologie
Volume 29, Number 6, 1998
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Page(s) | 547 - 554 | |
DOI | https://doi.org/10.1051/apido:19980607 |
Apidologie 29 (1998) 547-554
DOI: 10.1051/apido:19980607
Department of Animal Science, Agricultural University of Norway, P.O. Box 5025, 1432 Ås, Norway
Key words: biopsy / honeybee embryo / nuclear transplantation / embryo cloning / cryopreservation
DOI: 10.1051/apido:19980607
Biopsied preblastoderm honeybee embryos develop into normal honeybee queens
Ronglin Yu, Arne Hagen and Stig W. OmholtDepartment of Animal Science, Agricultural University of Norway, P.O. Box 5025, 1432 Ås, Norway
Abstract - Preblastoderm honeybee (Apis mellifera) embryos (8.5-9.0 h) were biopsied by extracting a small amount of ooplasm from the anterior part. Nearly 60 % of the embryos hatched into larvae and 45 % of these emerged as queens. It is shown that extraction of up to 80 nuclei is not likely to cause any morphological or behavioural abnormality in the adult queen. Beyond this number the survival rate declines rapidly. Combined with a technique for cryopreservation of ooplasmic fractions recently developed by us, we are now able to perform comprehensive testing or screening of adult honeybee queens, while having their totipotent embryonic nuclei stored in liquid nitrogen. © Inra/DIB/AGIB/Elsevier, Paris
Key words: biopsy / honeybee embryo / nuclear transplantation / embryo cloning / cryopreservation