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Table II
DNA amplified fragments to study variation in Varroa destructor samples. Amplified gene fragment, product size base pairs (bp) and annealing temperatures (Ta) are indicated. PCR primers defined based on the complete mitochondrial genome sequence of V. destructor (Navajas et al., 2002).
| Fragment | Primer name | Primer sequences (5’-3’) | Size (bp) | Ta (°C) | |
|---|---|---|---|---|---|
| cox1 | 10KbCOIF1 | CTT GTA ATC ATA AGG ATA TTG GAAC | 929 | 51 | |
| 6,5KbCOIR | AAT ACC AGT GGG AAC CGC | ||||
| atp6-cox3 | 16KbATP6F | GAC ATA TAT CAG TAA CAA TGAG | 818 | 51 | |
| 16KbCOIIIR | GAC TCC AAG TAA TAG TAA AACC | ||||
| cytb | 10KbCytbF-1 | GCA GCT TTA GTG GAT TTA CCT AC | 985 | 52 | |
| 10KbCytbPRIM | CTA CAG GAC ACG ATC CCA AG | ||||
| cox1* | C1 | GCG GTT CCC ACT GGT ATT | 458 | 52 | |
| C1R | AAT ACC AGT GGG AAC CGC | ||||
*
Fragment partially included in the 929 nucleotides long cox1 sequence and used here to sort out samples according to the published haplotypes (Anderson and Trueman, 2000).