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Table I
Treatments with respective total cell counts from pooled samples and percentage of viable cells listed in descending order of cell viability. Different letters in the % viable column indicate a significant difference (P < 0.002). Vit=vitrified freezing, Pro=programmable freezing, slow vs. rapid cool is treatment of sample prior to freezing.
| Treatment | Total cell counts | % Viable (+/–) SD1 |
|---|---|---|
| Pro/Slow cool/Harbo’s | 1403 | 92.94 +/– 0.87 (A) |
| Vit/Slow cool/Harbo’s | 626 | 77.48 +/– 1.67 (B) |
| Vit/Rapid cool/Harbo’s | 538 | 75.46 +/– 1.86 (B) |
| Vit/Rapid cool/HH DMSO | 755 | 52.85 +/– 1.82 (C) |
| Pro/Rapid cool/HH DMSO | 345 | 48.41 +/– 2.67 (D) |
| Vit/Slow cool/HH DMSO | 748 | 47.06 +/– 1.78 (E) |
| Vit/Slow cool/Extra DMSO | 1202 | 42.68 +/– 1.41 (E) |
| Vit/Rapid cool/Extra DMSO | 721 | 42.44 +/– 1.86 (E) |
| Pro/Slow cool/Extra DMSO | 1160 | 36.12 +/– 1.38 (F) |
| Vit/Slow cool/EG | 839 | 27.29 +/– 1.54 (G) |
| Pro/Slow cool/HH DMSO | 566 | 24.56 +/– 2.34 (G) |
| Pro/Rapid cool/EG | 507 | 22.29 +/– 2.07 (G) |
| Pro/Slow cool/EG | 896 | 14.29 +/– 1.19 (H) |
| Pro/Rapid cool/Harbo’s | 425 | 13.41 +/– 1.71 (H) |
| Vit/Rapid cool/EG | 492 | 2.44 +/– 0.68 (I) |